Matthew James Revington
NMR Facility Coordinator
Department of Chemistry and Biochemistry
University of Windsor
Phone: (519)253-3000, Ext 3997
Email: mrevingt@uwindsor.ca
Employment
- 2008-present
NMR Facility Co-ordinator
Department of Chemistry and Biochemistry University of Windsor, Windsor, Ontario, Canada
- 2007-2008
NMR Staff Scientist
Department of Structural Biology, St Jude Children’s Research Hospital, Memphis, Tennessee, USA
- 2004-2007
Research Fellow
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada
Supervisor: Dr. Gary Shaw
Project: NMR Protein Structural Determinations as part of the Ontario Centre for Structural Proteomics
- 2001-2004
Post-Doctoral Fellow
Biophysics Research Division, University of Michigan Ann Arbor, Michigan, USA
Supervisor: Dr. Erik Zuiderweg
Project: NMR studies of the Allosteric Mechanism of Thermus Thermophilus DnaK
Education
- PhD: Biochemistry, University of Western Ontario
Thesis: Structural Studies of the b subunit of Escherichia coli ATP synthase
Supervisors: Dr. Stanley D. Dunn and Dr. Gary S. Shaw - MSc: Medical Biophysics, Ontario Cancer Institute, University of Toronto
Thesis: NMR Studies of the Escherichia coli trp Repressor Complex with Operator DNA
Supervisor: Dr. Cheryl H. Arrowsmith - BSc: Chemistry and Biochemistry, University of Windsor, Windsor, Ontario, Canada
Teaching Experience
- Advanced NMR Workshop, Dept Chemistry and Biochemistry, University of Windsor 2011 and 2012, Created, Lectured and Facilitated
- Introductory NMR Workshop, Dept Chemistry and Biochemistry, University of Windsor 2010,2011 and 2012, Created, Lectured
- Teaching Assiatant 3rd year Biochemistry Laboratory, 1996-2000,
Department of Bicochemistry, University of Westen Ontario
Publications
- Crippen, G.M., Rousaki A., Revington M., Zhang Y. and . Zuiderweg E.R.P.
SAGA: rapid automatic mainchain NMR assignment for large proteins
J Biomol NMR 2010
- Bhattacharya, A., Revington, M., and Zuiderweg, E.R.P., Measurement and interpretation of 15N-1H residual dipolar couplings in larger proteins,
J Mag Res, 2009,.
- Malik, S. Revington, M., Smith, S. and Shaw, G.S.
Analysis of the structure human apo-S100B at low temperature indicates an unimodal conformational, distribution is adopted by calcium-free S100 proteins
Proteins: Structure, Function, and Bioinformatics (2008), 73, 28-42.
- Revington, M., Semesi, A., Yee, A., Arrowsmith, C.H., and Shaw, G.S.
The solution structure of E. coli ydhA, a periplasmic β-barrel protein
J. Biomol. NMR (2006), 35, 295-300
Revington, M., Semesi, A., Yee, A., Shaw, G.S.
- Solution structure of the{ the} Escherichia coli protein ydhR: a putative mono-oxygenase
Protein Sci. (2005) 14, 3115-3120
- Revington, M., Zhang, Y., Yip, G.N.B., Kurochkin, A.V., and Zuiderweg, E.R.P.
NMR investigations of allosteric processes in a two-domain Thermus Thermophilus DnaK molecular chaperone
J. Mol. Biol. (2005), 349, 163-183
- Revington, M., Holder, T.M., and Zuiderweg, E.R.P.
NMR study of nucleotide-induced changes in the nucleotide-binding domain of Thermus Thermophilus Hsp70 chaperone DnaK: Implications for the allosteric mechanism
J. Biol. Chem. (2004), 279, 33958-339678. - Revington, M., and Zuiderweg, E.R.P.
TROSY-driven NMR backbone assignments of the 381-residue nucleotide-binding domain of the Thermus Thermophilus DnaK molecular chaperone
J. Biomol. NMR, (2004), 30,113-114
- Lee, C.L., Revington, M., Dunn, S.D., and Petersen, N.O.
The Lateral Diffusion of Selectively Aggregated Peptides in Giant Unilamellar Vesicles,
Biophys. J. (2003), 85,1338
- Revington, M., Dunn, S.D., and Shaw, G.S.
Folding and stability of the b subunit of the F(1) F(0) ATP synthase.
Protein Sci. (2002), 11, 1227-1238
- Dunn, S.D., Bi, Y., and Revington, M.
A Re-examination of the Structural and Functional Consequences of Mutation of Alanine-128 of the b subunit of Escherichia coli ATP Synthase to Aspartic Acid.
Biochim. Biophys. Acta (2000) 1459, 521-527
- Dunn, S.D., Revington, M., Cipriano, D.J., and Shilton, B.H.
The b Subunit of Escherichia coli ATP Synthase.
J. Bioenerg. Biomem., (2000), 32, 347-355
- Dunn, S.D., McLachlin, D.T., and Revington, M.
The Second Stalk of Escherichia coli ATP Synthase.
Biochim. Biophys. Acta, (2000), 1458, 356-363
- Revington, M., McLachlin, D.T., Shaw, G.S., and Dunn, S.D.
The Dimerization Domain of the b Subunit of the Escherichia coli F1F0-ATPase.
J. Biol. Chem., (1999), 274, 31094-31101
- Lee, W., Revington, M., Farrow, N.A., Nakamura, A., Utsunomiya-Tate, N., Miyake, Y., Kainosho, M., and Arrowsmith, C.H.
Rapid corepressor exchange from the trp-repressor/operator complex: an NMR study of [ul-13C/15N]-L-tryptophan.
J. Biomol. NMR., (1995), 5, 367-375
- Yamazaki, T., Lee, W., Revington, M., Mattiello, D.L., Dahlquist, F.W., Arrowsmith, C.H., and Kay, L.E.
An HNCA Pulse Scheme for the Backbone Assignment of 15N,13C,2H-Labelled Proteins: Application to a 37-kDa Trp Repressor-DNA Complex
J. Am. Chem. Soc.,(1994), 116, 6464-6465
- Lee., W., Revington, M.J., Arrowsmith, C., and Kay, L.E.
A pulsed field gradient isotope-filtered 3D 13C HMQC-NOESY experiment for extracting intermolecular NOE contacts in molecular complexes.
FEBS Lett., (1994), 350, 87-90
- Zhang, H., Zhao, D., Revington, M., Lee, W., Jia, X., Arrowsmith, C., and Jardetzky, O.
The solution structures of the trp repressor-operator DNA complex
J. Mol. Biol., (1994), 238, 592-614
Book Chapters
- Revington, M.J., Lee, W., and Arrowsmith, C.H.
Heteronuclear Strategies for the Assignment of Larger protein/DNA complexes: Application to the 37 kDa trp Repressor- Operator Complex, Biological NMR Spectroscopy, Edited J. Markley and S. Opella, Oxford Press, (1997), p50-66
- Arrowsmith, C.H., Lee, W., Revington, M., Yamazaki, T., and Kay, L.E.
Towards the Solution Structure of Large (>30kDa) Proteins and Macromolecular Complexes, Techniques in Protein Chemistry VI, Academic Press, (1995), p503-547